Figure 1

A diagram of the experimental apparatus and the topographical arrangement of the sonication path and electrodes. (A) The transducer was mounted on a 3-axis stereotactic positioning system and submerged in degassed water. The coordinates of the acoustic focus were controlled under the metric guidance of the stereotactic coordinates of the rat brain, which were marked on a plastic plate. After localization of the acoustic focus, the animal was laid supine on a plastic tray that was mounted above the system. The head was partially submerged in an oval hole that opened into a bag of degassed water in order to secure an uninterrupted beam profile from the transducer to the targeted tissue. (B) Schematic diagram of the positions of bilateral subdermal EEG electrodes (marked as small blue circles) relative to the area of the sonication beam path (marked as a red circular boundary) on the rat skull as well as to the sonication focus (marked as a solid red circle).