Brain lipid analysis of Fa2h-/-/ Cgt-/- mice. (A) TLC analysis of sphingolipids (after mild alkaline hydrolysis) from total brain of 4-week-old animals of the indicated genotypes showed a strong reduction of GlcCer in Fa2h-/-/Cgt-/- (lane 3) compared to Cgt-/- mice (lane 2). The amount of lipids isolated from 0.05 mg of tissue (wet weight) was loaded per lane. LPE, lyso-phosphatidylethanolamine; SM, sphingomyelin. (B) TLC analysis of total brain lipids (not subjected to alkaline hydrolysis). In order to demonstrate the presence of NFA-GlcCer in Fa2h-/-/Cgt-/- mice, the amounts of lipids was increased 4-fold in case of Fa2h-/-/Cgt-/- mice.