Figure 9From: Kalrn promoter usage and isoform expression respond to chronic cocaine exposureEffect of chronic exposure to cocaine on expression of Kalirin protein. A. Striata harvested from Saline and Cocaine treated mice were subjected to subcellular fractionation; equal amounts (10 μg) of input (S1) and PSD were fractionated by SDS-PAGE. Kalirin proteins were visualized using the Kal-spectrin antibody. DARPP32, a soluble protein highly expressed in the striatum, was excluded from the PSD fraction. B. Striata prepared in a similar fraction to A show increases in Kal7 in the S1 and PSD fractions while demonstrating a lack of Kal7 in the Triton soluble fraction as was expected from previous results [17, 18]. C. Levels of Kal7 and Kal9 in SDS lysates prepared from the nucleus accumbens of chronically Saline and Cocaine treated mice were compared to levels of βIII-tubulin; Kal12 levels were not high enough to allow accurate quantification. The Kalirin/Tubulin ratios for Kal7 and Kal9 were calculated for Saline and Cocaine treated mice, with the Saline ratio normalized to 100%; using an unpaired t-Test, Kal7 expression (p < 0.05) differed significantly while Kal9 expression did not.Back to article page