Plots showing patterns of hair cell and hearing loss as a function of exposure frequency. (A) Effects of tone exposure frequency and location along the rostral-caudal axis on percentage of hair cell bundle loss for each of the 19 predetermined locations for both left and right saccules of each fish (N = 819). Along the rostral-caudal axis, 1 (150 μm) is most rostral and 7 (1650 μm) is most caudal. (B) Effects of tone exposure frequency and hearing threshold frequency on hearing loss (threshold shifts) for each of the 11 hearing frequencies tested (N = 264). Plots (A) and (B) were rotated 180° to produce (C) and (D), respectively. Three-dimensional surfaces were constructed by distance-weighted least-squares smoothing of the original data, which imposes no a priori relationships between the variables.