Comparison between control and tone-exposed saccules at three different locations along the rostral-caudal axis. Phalloidin-labeled saccular epithelia showing evidence of differential hair cell bundle loss between control (A, C, E) and tone-exposed (B, D, F = 2000, 800, 100 Hz exposures, respectively) saccules. A and B, C and D, and E and F show saccular locations 7-9, 10, and 14-16, respectively. 50 × 50 μm counting boxes are shown. X's were used to mark counted hair cell bundles. Scale bars = 100 μm.