Table 2 Primers used in cloning, chromosomal mapping and real-time RT-PCR for porcine Dab1 and Fyn isoforms
From: Identification of alternatively spliced Dab1 and Fyn isoforms in pig
Primers | Application | Size(bp) | Sequence (5' to 3') |
|---|---|---|---|
P1 | Porcine Dab1 cloning | 1867/1750 | 5'-ATGTCAACTGAGACAGAACTTCAAGTAGC-3' |
P2 | Â | Â | 5'-TCCCTCTTCCAAGCGAGTTCC-3' |
P3 | Porcine Fyn cloning | 1614/1605 | 5'-ATGGGCTGTGTGCAATGTAAGGA-3' |
P4 | Â | Â | 5'-TTACAGGTTTTCACCGGGTTGATAC-3' |
P5 | sDab1 mapping | 321 | 5'-ATGACCGACTGGGCTTACTGTTC-3' |
P6 | Â | Â | 5'-CCAAATCACACAGCTTGAAAGAGTC-3' |
P7 | sFyn mapping | 261 | 5'-CTGCCTCAGATTTCCAGACGC-3' |
P8 | Â | Â | 5'-AGCAGGATGAGGTCAGGAGCC-3' |
P9 | sDab1 expression | 207 | 5'-CCATCCAAATCATCTGCATCCC-3' |
P10 | Â | Â | 5'-GACCTGTGCTATCTAGCTACCGGC-3' |
P11 | sDab1-Li expression | 176 | 5'-GTATCAGGTTCCCACCAGCCAA-3' |
P12 | Â | Â | 5'-GGGTCACAGCCTTTGTTGCCT-3' |
P13 | sFyn-B expression | 179 | 5'-AAAGGGATGCCAAGGCTTACC-3' |
P14 | Â | Â | 5'-GACATTGTGCCTGGTTTGAGAGTC-3' |
P15 | sFyn-T expression | 186 | 5'-AACTGTGATTGCATCGAGTTGTACC-3' |
P14 | Â | Â | 5'-GACATTGTGCCTGGTTTGAGAGTC-3' |
P17 | GAPDH expression | 220 | 5'-GCAAATTCCACGGCACAGTCA-3' |
P18 | Â | Â | 5'-TCAGCAGAAGGGGCAGAGATG-3' |