Skip to main content

Advertisement

Table 2 Primers used in cloning, chromosomal mapping and real-time RT-PCR for porcine Dab1 and Fyn isoforms

From: Identification of alternatively spliced Dab1 and Fyn isoforms in pig

Primers Application Size(bp) Sequence (5' to 3')
P1 Porcine Dab1 cloning 1867/1750 5'-ATGTCAACTGAGACAGAACTTCAAGTAGC-3'
P2    5'-TCCCTCTTCCAAGCGAGTTCC-3'
P3 Porcine Fyn cloning 1614/1605 5'-ATGGGCTGTGTGCAATGTAAGGA-3'
P4    5'-TTACAGGTTTTCACCGGGTTGATAC-3'
P5 sDab1 mapping 321 5'-ATGACCGACTGGGCTTACTGTTC-3'
P6    5'-CCAAATCACACAGCTTGAAAGAGTC-3'
P7 sFyn mapping 261 5'-CTGCCTCAGATTTCCAGACGC-3'
P8    5'-AGCAGGATGAGGTCAGGAGCC-3'
P9 sDab1 expression 207 5'-CCATCCAAATCATCTGCATCCC-3'
P10    5'-GACCTGTGCTATCTAGCTACCGGC-3'
P11 sDab1-Li expression 176 5'-GTATCAGGTTCCCACCAGCCAA-3'
P12    5'-GGGTCACAGCCTTTGTTGCCT-3'
P13 sFyn-B expression 179 5'-AAAGGGATGCCAAGGCTTACC-3'
P14    5'-GACATTGTGCCTGGTTTGAGAGTC-3'
P15 sFyn-T expression 186 5'-AACTGTGATTGCATCGAGTTGTACC-3'
P14    5'-GACATTGTGCCTGGTTTGAGAGTC-3'
P17 GAPDH expression 220 5'-GCAAATTCCACGGCACAGTCA-3'
P18    5'-TCAGCAGAAGGGGCAGAGATG-3'