Validation of regional enrichment by QPCR. Relative gene expression as demonstrated by TaqMan QPCR for 20 selected genes enriched in rat A) FMCx, B) TCx and C) OCx. The diagrams display mean values for up to twelve replicates from each region, including left and right hemispheres from the three rats included in the microarray study as well as three additional rats included for validation purposes. Gene expression levels in the samples from the three additional rats completely correlated with data from the original rats. ANOVA (p < 0.05) confirmed significant regional enrichment across cortical regions for all genes, with p-values ranging from 10-5 - 10-11 for the FMCx genes, 10-3 - 10-12 for the TCx genes and 10-2 - 10-6 for the OCx genes. Expression of Crim1 and Atoh7 could not be detected by their respective TaqMan assays. Details on TaqMan assays and statistical results are included in Additional file 4. FMCx, fronto-medial cortex; TCx, temporal cortex; OCx, occipital cortex.