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Table 1 Electrophysiological properties of chemically identified neurons in the rat medial septal/diagonal band complex

From: The nestin-expressing and non-expressing neurons in rat basal forebrain display different electrophysiological properties and project to hippocampus

  nestin+ nestin- & ChAT+ GAD67+ VGLUT+
number of neurons 33 32 13 9
Cm (pf) 55.76 ± 3.05 b 49.60 ± 2.37 b 76.99 ± 9.60 43.58 ± 3.92 b
Rm (MΩ) 400.56 ± 26.86 387.26 ± 27.27 289.63 ± 44.25 570.07 ± 120.25
Tau (ms) 0.85 ± 0.07 0.70 ± 0.05a 1.27 ± 0.18 0.64 ± 0.06 a
RMP (mV) -56.73 ± 1.05 -59.75 ± 1.14 -55.62 ± 1.91 -58.00 ± 3.47
AHP duration (ms) 223.77 ± 12.08 b 230.52 ± 8.48 b 165.23 ± 7.04 238.17 ± 16.97 a
AHP amplitude (mV) 4.49 ± 0.21 b 5.02 ± 0.35 b 3.27 ± 0.25 4.80 ± 0.28 b
AP amplitude (mV) 99.04 ± 1.37 a 97.39 ± 1.22 b 104.97 ± 2.22 98.85 ± 2.82
Spike half width (ms) 0.88 ± 0.04 b 0.94 ± 0.03 b 0.67 ± 0.06 0.99 ± 0.05 b
Spike width (ms) 2.46 ± 0.09 b 2.66 ± 0.06 b 2.02 ± 0.14 2.78 ± 0.14 b
rise time (ms) 0.30 ± 0.01 b 0.32 ± 0.01 b 0.23 ± 0.02 0.32 ± 0.01 b
rise slope (mV/ms) 121.91 ± 8.30 105.85 ± 4.99 a 196.45 ± 24.41 106.31 ± 8.06 a
decay time (ms) 0.69 ± 0.03 b 0.73 ± 0.03b 0.51 ± 0.05 0.82 ± 0.06 b
decay slope (mV/ms) -49.57 ± 3.21 a -43.69 ± 1.81 b -83.06 ± 9.41 -38.98 ± 2.55 b
MF (Hz) 8.39 ± 0.45 b 8.19 ± 0.49 b 18.38 ± 1.73 9.22 ± 0.97 b
FMAX (Hz) 20.73 ± 2.07 a 18.86 ± 1.77 b 28.99 ± 1.93 19.83 ± 0.82 b
FSTEADY (Hz) 6.86 ± 0.53 b 6.67 ± 0.35 b 17.23 ± 1.66 7.01 ± 0.49 b
spike adaptation 0.55 ± 0.05 b 0.56 ± 0.04 b 0.28 ± 0.06 0.56 ± 0.11 a
depolarizing sag (mV) 2.74 ± 0.62 b 1.81 ± 0.32 b 16.81 ± 0.77 2.22 ± 0.56 b
Ih (pA) -12.90 ± 2.57 b -4.00 ± 2.17 b -186.82 ± 15.73 -3.49 ± 1.86 b, c
  1. Nestin+, nestin mRNA-positive neuron (including nestin mRNA-positive & ChAT mRNA-negative neurons and nestin mRNA-positive & ChAT mRNA-positive neurons); Nestin- & ChAT+, nestin mRNA-negative and choline acetyl transferase mRNA-positive neuron; GAD67+, glutamic acid decarboxylases 67 mRNA-positive neuron; VGLUT+, vesicular glutamate transporter 1 or/and 2 mRNA-positive neuron. Cm, membrane capacitance; Rm, membrane resistance; Tau, time constant; RMP, resting membrane potential; threshold, action potential threshold; AHP, afterhyperpolarization; MF, Mean firing rate; FMAX, maximal firing frequency; FSTEADY, steady firing frequency. Ih, hyperpolarization activated current of neurons when hyperpolarized from -50 to -120 mV. The current pulses used for membrane property analyses were 0.1-0.3 nA. All values are presented as means ± S.M.E. Significant differences are identified: a versus GAD67+ neurons, P < 0.05; b versus GAD67+ neurons, P < 0.01; c versus nestin+ neurons, P < 0.05.