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Figure 1 | BMC Neuroscience

Figure 1

From: The nestin-expressing and non-expressing neurons in rat basal forebrain display different electrophysiological properties and project to hippocampus

Figure 1

Nestin mRNA-positive cells in MS/DBB are functional neurons. A. Agarose gel analysis of the sc-RT-PCR products obtained from a single MS/DBB cell. The only PCR-generated fragment was nestin. B. Whole cell current of the same cell depolarized from -60 mV to 10 mV in voltage-clamp mode showed characteristic of functional neurons. C. The cell shows typical neural action potential when depolarized to the threshold potential. D. A sustained depolarizing current (1000 ms) elicits a train of repetitive action potentials. The membrane responses were elicited using positive currents of 0.2 nA from -60 mV in panel C and D.

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