TRPM8 expression in ipsilateral L5 DRGs of CCI and sham-operated rats. A, TRPM8 protein staining in L5 DRG sections. Arrows indicate TRPM8 positive neurons. The graph shows the percentage of TRPM8 positive neurons. B, TRPM8- immunoblotting of L5 DRGs. β-actin expression is shown as a control. The graph shows the ratio of density of TRPM8 band to that of β-actin band. Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01 vs. sham-operated rats (t test). Magnification of immunohistochemical images is 400 ×, and scale bars represent 25 μm.