Table 2 Secondary antibodies used for immunohistochemistry
Conjugate and host | Against | Dilution | Supplier |
|---|---|---|---|
FITC (goat) | anti-rabbit | 1:100 | Cayman Chemical, Ann Arbor, MI |
Cy2 (donkey) | anti-rabbit | 1:200 | Jackson Immuoresearch, West Grove, PA |
Texas-Red (donkey) | anti-rabbit | 1:200 | Jackson Immuoresearch, West Grove, PA |
Alexa 488 (donkey) | anti-goat | 1:400 | Invitrogen, CA |
Cy2 (donkey) | anti-goat | 1:200 | Jackson Immuoresearch, West Grove, PA |
Cy3(donkey) | anti-goat | 1:200 | Jackson Immuoresearch, West Grove, PA |
Texas-Red (donkey) | anti-mouse | 1:200 | Jackson Immuoresearch, West Grove, PA |
Texas-Red (donkey) | anti-sheep | 1:200 | Jackson Immuoresearch, West Grove, PA |