CGRP and synaptophysin expression in human C1. (A) Double-staining of CGRP (red) and synaptophysin (green) in human C1. Co-localization is shown in the merged picture. (B) Detailed confocal image showing staining of CGRP (red, thin arrow) and synaptophysin (green, thick arrow). Co-localization (yellow, arrow heads) is demonstrated in the fibers. (C) Synaptophysin and DAPI staining. "Pearl-like" synaptophysin immunoreactivity (green) is detected in all laminae in the fibers within C1-level.