CLR/RAMP1 and CGRP double-staining in human STN. (A) Expression of RAMP1 in human STN. The expression is found in the spinal trigeminal tract region (arrow). (B) Co-expression of the receptor components, CLR (green) and RAMP1 (red), in fibers in the spinal trigeminal tract (arrows). (C) Confocal microscopy showing expression of CGRP (red, arrows) and RAMP1 (green, arrows). CGRP immunoreactivity is found in the superficial laminae, while the receptor component is expressed in the spinal trigeminal tract. No co-localization is found. Asterisks point at autofluorescent lipofuscin.