Double-staining of CGRP and MBP or synaptophysin (synaptic vesicles). (A, B) Double-staining of CGRP (red) and MBP (green) in rat STN. Nuclei staining (DAPI, blue) is used in the merged pictures. Arrows point at CGRP positive areas negative for MBP. (C) CGRP (red) and synaptophysin (green) expression (arrows). Note that not all CGRP positive fibers co-localize with synaptophysin (arrow heads).