Figure 5

P2X4 expression and phenotype change in primary cultured amoeboid microglial cells after the hypoxic exposure with PPADS or TNP-ATP treatment. By immunofluorescence labeling, P2X4 is colocalized with OX42 in the primary cultured microglial cells (Aa-c, Scale bar = 20 μm). Following hypoxia, the phenotype of primary cultured AMC undergoes alteration (Ba-c, Scale bar = 20 μm) in which the cell body becomes rounded; however, the cells show long extending processes after treatment with PPADS (Ca-c, Scale bar = 20 μm). A more obvious change was found in hypoxic microglial cells treated with TNP-ATP (Da-c, Scale bar = 20 μm).