P2X4 expression by western blotting in P0 rats subjected to hypoxia (Hy) at different time points in corpus callosum (CC). A. P2X4 expression after hypoxia in P0 rats. P0 rats exposed to Hy and sacrificed at 4 (Hy4 h), 24 (Hy24 h), 72 (Hy72 h), 7D (Hy7 d) and 14D (Hy14 d). It shows P2X4 (60 kDa) and β-actin (42 kDa) immunoreactive bands, respectively. P2X4 expression in CC after hypoxia is noticeably enhanced in the hypoxic rats at Hy4 h, Hy24 h, Hy72 h and Hy7 d when compared with the matching controls. B. Quantitative analysis of P2X4 expression after hypoxia in CC. P2X4 protein express in the CC at 3, 24, 72h, 7 and 14 d after the hypoxic exposure in P0 rats. In bar graph, relative P2X4 protein levels were normalized to β-actin levels in control and hypoxia groups. Data are mean ± SD, n = 6. *P < 0. 05 or **P < 0. 01 hypoxia vs. with their corresponding controls.