Anterograde BDA-Labelled Fibres in the Spinal Cord of Saline-Treated Animals. A) Montage of SCI epicentre (black asterisk) demonstrating extensive anterograde labelling of ascending fibres in both proximal and distal stumps of the injured spinal cord (black arrows). B-G) Higher magnification images confirming the presence of BDA-labelled fibres extending across both stumps (black arrows), consistent with previous retrograde labelled results described in Figure 2. H) Quantification of percentage of BDA-labelled fibres revealed the presence of regenerated ascending fibres found 1 mm rostral and at the injury epicentre (0 mm) in control animals only (***P < 0.0001). Similar number of labelled fibres was found 1 mm caudal in the spinal cord of control and liposome-treated animals. BDA = biotinylated dextran amine, DCC = dorsal column cut. Directional key: D = dorsal, V = ventral, C = caudal, R = rostral. Scale bars montage 800 μm (horizontal bar) and 1 mm (vertical bar), B -E 200 μm, F-G enlarged views 100 μm.