Skip to main content

Advertisement

Table 3 Contractile effects of ET-1 and 5-CT in MCA and basilar arteries

From: Inhibition of cerebrovascular raf activation attenuates cerebral blood flow and prevents upregulation of contractile receptors after subarachnoid hemorrhage

    Biphasic curve Sigmoidal curve
  N K+ mean ±
s.e.m
Emax 1(%) ±
s.e.m
Emax 2(%) ± s.e.m pEC50(1) ±
s.e.m
pEC50(2) ±
s.e.m
Emax (%) ±
s.e.m
pEC50 ±
s.e.m
ET-1 MCA         
Sham 6 1.76 ± 0.57      152 ± 11 8.59 ± 0.14
SAH 7 1.95 ± 0.34 28 ± 8a, b 148 ± 5 12.62 ± 0.23a, b 9.35 ± 0.22   
SAH+SB386023-b at 0 h 5 2.22 ± 0, 35      133 ± 5 8.87 ± 0, 15
SAH+SB386023-b after 6 h 9 1.81 ± 0, 27      176 ± 6 8.92 ± 0.13
SAH+SB386023-b after 12 h 5 1.45 ± 0.27 27 ± 7a, b 149 ± 17 12.02 ± 0.39a, b 9.13 ± 0.17   
ET-1 BA         
Sham 6 4.00 ± 0.58      119 ± 9 9.37 ± 0.10
SAH 6 3.70 ± 0.47 34 ± 5a, b 126 ± 8 12.21 ± 0.15a, b 9.67 ± 0.02   
SAH+SB386023-b at 0 h 6 3.92 ± 0.45      124 ± 5 9.23 ± 0.16
SAH+SB386023-b after 6 h 6 4.23 ± 0.41      155 ± 10 8.80 ± 0.13
SAH+SB386023-b after 12 h 6 3.75 ± 0.32 29 ± 5 125 ± 5 12.41 ± 0.18 9.75 ± 0.23   
5-CT MCA         
Sham 6 1.67 ± 0.54 17 ± 6a 42 ± 9a 8.40 ± 0.14 6.08 ± 0.64a   
SAH 6 2.35 ± 0.40 35 ± 2a, b 60 ± 3a, b 8.47 ± 0.17 7.11 ± 0.01a, b   
SAH+SB386023-b at 0 h 5 2.45 ± 0.25 16 ± 5b 43 ± 8 8.48 ± 0.13 6.39 ± 0.33   
SAH+SB386023-b after 6 h 6 1.81 ± 0.27 21 ± 5b 45 ± 17 8.43 ± 0, 11 6.33 ± 0.51   
SAH+SB386023-b after 12 h 6 2.09 ± 0.35 50 ± 6 92 ± 9 8.37 ± 0.10 6.47 ± 0.02   
5-CT BA         
Sham 6 3.90 ± 0.62 18 ± 6a 56 ± 9 8.00 ± 0.11a 6.25 ± 0.15   
SAH 8 4.00 ± 0.26 43 ± 6a, b 64 ± 5 8.46 ± 0.21a, b 6.68 ± 0.01   
SAH+SB386023-b at 0 h 6 3, 89 ± 0.22 20 ± 4b 47 ± 4 8.09 ± 0.21a 6.12 ± 0.12   
SAH+SB386023-b after 6 h 6 4.23 ± 0.41 12 ± 3b 47 ± 9 7.92 ± 0.23b 6.05 ± 0.44   
SAH+SB386023-b after 12 h 6 3.75 ± 0.43 38 ± 5b 55 ± 4 8.30 ± 0.19a, b 6.48 ± 0.10   
  1. Responses were characterized by Emax values, expressed as percent of 63 mM K+ -induced contraction, and pEC50 values (negative logarithm of the molar concentration that produces half maximum contraction). Values are represented as mean ± s.e.m and n represents number of animal. a = significant difference between SAH and control groups, b = significant difference between SAH and SAH treated with SB386023-b