CIA reduces amyloid plaque burden in APP/PS1 mice. (A) Bright field photomicrographs of brain sections showing amyloid plaques immunolabeled with 4G8 in 6-month-old wild-type mice (Tg-vehicle) and APP/PS1 mice treated with vehicle (Tg+ vehicle) or CIA (Tg+ CIA). Scale bar = 2 mm. (B) Plaque burden was analyzed by measuring areas positive for 4G8 (Tg+ vehicle, n = 3; Tg+ CIA, n = 4) or thioflavine-S (Tg+ vehicle, n = 7; Tg+ CIA, n = 8). (C) ELISA of soluble and insoluble forms of Aβ40 and Aβ42 in combined cortical and hippocampal tissues (Tg+ vehicle, n = 7; Tg+ CIA, n = 8).