Ca2+-transients in flies with pharmacologically blocked RyR or knocked-down RyR. Response profiles of ryanodine-treated flies (A-C), or Or83b,GA/RyR-RNAi expressing flies (D-F) to 5 s application of spearmint, citronella, or octanol, applied 5 time, at 5 min-intervals. The decrease of the amplitude of the successive evoked transients was not significant in the presence of ryanodine for the 3 odors, contrary to that for the RyR-RNAi flies. (G-I) Mean of the total photons for each condition. (J-L) Mean of the duration of the response (s). n = 5-9 flies for each condition. Values are means +/- SEM. Statistics: same tests as for Figure 2 (see Table 1 for statistical values).