Ca2+-transients in flies with pharmacologically blocked InsP
R or knocked-down InsP
R. Response profiles of thapsigargin-treated flies (A-C), or Or83b,GA/InsP3R-RNAi flies (D-F) to 5 s application of spearmint, citronella, or octanol, applied 5 times at 5 min-interval. Although there is a tendency to decrease, the amplitude of evoked-activity during the repeated stimulations was not significantly different in the presence of thapsigargin or in InsP3R-RNAi-expressing flies for the 3 odors, except for spearmint and octanol in these latter flies. (G-I) Mean of the total photons for each condition. (J-L) Mean of the duration of the response (s). n = 5-9 flies for each condition. Values are means +/- SEM. Statistics: same tests as for Figure 2 (see Table 1 for statistical values).