The second component of the Ca2+ -response depends on cholinergic synaptic transmission. Mean (+/- SEM) amplitude of the overall responses (photons/s versus time) of the effect of α-bungarotoxin (Bgt) (A,B,C) on the Ca 2+-induced responses collected from the ROI, evoked by 5 application at 5-min interval, of a 5 s odor-duration of spearmint, citronella or octanol. (G-I) Mean of the total photons for each odor. (J-L) Mean of the duration(s) of the response. (D,E,F) Magnification view of the first odor application, showing the superposition of the Bgt-treated versus control flies (issued from Figure 2D,E,F respectively) for each odor. n = 5-9 flies for each condition. For the histograms (G-L): Values are means +/- SEM. Statistics: same tests as for Figure 2 (see Table 1 for statistical values).