Cerebellar architecture and morphology of Purkinje cells in Alpk1PB/PB mice. (A, B) H&E staining of the cerebellar cross sections from 6-week-old Alpk1PB/PB mice and their wild type littermates. No gross abnormalities were found in the Alpk1PB/PB mice. Scale bar: 500 μm. (C-F) Confocal images showing Purkinje cell morphology identified by anti-Calbindin immunofluorescence staining of the cerebellar slices from 6-week-old Alpk1PB/PB mice and their wild type littermates. Purkinje cell alignment and dendrite outgrowth (C, D) and Purkinje cell dendritic distal branches (E, F) displayed no obvious differencesbetween the Alpk1PB/PB mice and their wild type littermates. All images are representatives of five sections from at least 3 mice per genotype. Scale bar, C, D: 10 μm; E, F: 2 μm. (G-I) Statistic analysis of Purkinje cell morphology. High-resolution confocal image stacks of cerebellar slices from 6-week-old Alpk1PB/PB mice and their wild type littermates were processed by Image Pro Plus analysis. Main dendrite length (G), visible Purkinjce cells per scopeview (H) and area of Purkinje cell body (I) were quantified. Data are mean ± SEM of about 200 cells from several cerebellar slices of at least two individual mice per genotype. Experimenter was blind to the genotypes. **P < 0.01.