Expression pattern of ALPK1 in wild type and Alpk1PB/PB mice. (A) Immunoblots were performed on different tissues from adult wild type mice by using anti-ALPK1 antibody. ALPK1 short isoform (108 kD) was highly expressed in brain, spinal cord, heart, lung, spleen, thymus, small intestine, skin and testis, while detectable inskeletal muscle and kidney. ALPK1 large isoform (130 kD) appeared in heart, lung, thymus and skin.(B) Immunoblots were performed on brain and spinal cord extracts from different aged wild type mice. Only 108 kD isoform of ALPK1 was detected in mouse brain and spinal cord. P0, P7, P14 and P21 stand for postnatal day 0, 7, 14 and 21, respectively. 1 m: one month. (C) Immunoblots were performed on severaltissues from adultwild type (WT) and Alpk1PB/PB mice (HO)by using anti-ALPK1 antibody. In skeletal muscle, spleen, small intestine and thymus, the expression level of ALPK1 was dramatically decreased in Alpk1PB/PBmice, while in brain and spinal cord, the expression level of ALPK1 were increased. Br, brain;Sp.C, spinal cord; He, heart; Li, liver; Lu, lung; Sk.m, skeletal muscle; Sp, spleen; Th, thymus; Bo, bone; Ki, kidney; Sm.I, small intestine; Ski, Skin; Te, testis; M, protein marker.