Fluorescent immunostaining showing the localization of flAgRP versus VWF-AgRP
. Primary cortical neurons were infected with AAV-flAgRP(A) or AAV-VWF-AgRP83-132 (B) at a multiplicity of infection of 10.000. Seventy hours after infection neurons were fixed and immunostained for AgRP (green) and βIII-tubulin (red). "shows the overlay of green and red signals.