Table 4 Sequences of primers used for real-time PCR
Target Protein | Forward Primer | Reverse Primer | PCR Product Size (bp) | Sequence Accession No. |
|---|---|---|---|---|
CACN | 5'-TCACCATTGCCTCCGAACACTA-3' | 5'-CAGGAGCATTTCTGCCGTGA-3' | 104 | NM012517 |
TrkA/B | 5'-GCCACACAATGTTGCCCATC-3' | 5'-AAGGACTCTGCCCTGGGTGA-3' | 185 | NM012731 |
FGFR | 5'-TTGCCGAATGAAGACCACGA-3' | 5'-GGAGTTCATGGACGAGCTGGA-3' | 130 | NM001109892 |
PDGFR | 5'-GAATGACCACGGCGATGAGA-3' | 5'-GGATAAGCCTCAAACACCACCTG-3' | 141 | NM031525 |
G12 | 5'-CAAGATGCTGGTGGCTGTCAA-3' | 5'-AGCAGCTCTGCCTCACGATG-3' | 81 | NM021589 |
Raf1 | 5'- AACAGTGAAGTCGCGCTGGA-3' | 5'- CAGCACAATGCCATAGGAGTAGACA-3' | 145 | NM012639 |
MKP | 5'-ACAACCACAAGGCAGACATTAGCTC-3' | 5'- CAGATGGTGGCTGACCTGGA-3' | 127 | NM053769 |
JIP3 | 5'-AGCCTGCCTGCCAAGTACAAG-3' | 5'-TCAGGTTGACACCAGCAGCAC-3' | 148 | NM001100673 |
HGK | 5'-TTCACCATGTCATTCACCGAGA-3' | 5'-CCAGCTGAGCGCTTACACCA-3' | 99 | NM001106904 |
HSP72 | 5'-GCTTTCACCTCAAGCCTTTGGA-3' | 5'-CGGGCCTCATGCACACATAG-3' | 117 | NM212504 |
GAD D45 | 5'-AGGCAGCCAAGCTGCTCAA-3' | 5'-ACGTCCCGGTCGTCATCTTC-3' | 82 | NM024127 |
GAD D153 | 5'-TGGAAGCCTGGTATGAGGATCTG-3' | 5'-GAGGTGCTTGTGACCTCTGCTG-3' | 175 | NM024134 |
GAPDH | 5'-GGCACAGTCAAGGCTGAGAATG-3' | 5'-ATGGTGGTGAAGACGCCAGTA-3' | 143 | NM017008 |