Figure 2
From: Tri-partite complex for axonal transport drug delivery achieves pharmacological effect
Outline of conjugation and synthesis. A polymer such as dextran is first activated with cyanogen bromide to generate a carboxy modified dextran that can be conjugated to linker molecules attached at each monomer. The linkers are then conjugated with drug molecules. A second cyanogen bromide activation step is then carried out and followed by conjugation with an axonal transport facilitator molecule (ATF). An affinity purification step is then used to obtain drug loaded polymer complexes with ATF attached.