VGLUT1 immunoreactivity in TRPM5-GFP mice. The figures illustrate the expression of GFP under the control of the TRPM5 promoter (green), VGLUT1 (red) in circumvallate papillae (CV; A-C), fungiform papillae (FF; D-F). In circumvallate taste buds, VGLUT1 is not expressed in taste cells but only in nerve fibers surrounding these taste cells. Each figure represents merged images from a Z-series. The Photoshop digital filters Dust & Scratches and Unsharp Mask were used in some images to eliminate pixel noise in this and subsequent figures containing confocal images.