PN-1 expression increases after running wheel exercise. (A-B) PN-1 expression detected by X-Gal staining in the brain of a reporter knock-in mouse (PN-1KI) without (CTR) and after 12 days of running exercise (RUN). (C, D) Enlargement of the dentate gyrus in control and exercised animals, respectively. (E, F) Enlargement of the thalamus in control and exercise animals, respectively. (G, H) BrdU and X-Gal staining in controls and after running, respectively. BrdU-positive cells (brown) lack PN-1 (black arrows) or are positive for PN-1 (white arrows). Enlargements are shown on the upper left. Cx: Cortex; DG: Dentate gyrus; Th: Thalamus. Scale bars: A, B, G and H 800 μm (insert in H 400 μm), C, D 400 μm, E, F 100 μm.