Skip to main content

Table 3 Relative proportion of aNS-1 cells expressing neural progenitor, neuronal and glial antigens during differentiation in 96- and 384-well microplates

From: Adaptation of NS cells growth and differentiation to high-throughput screening-compatible plates

Plate format

Nestin

beta III-tubulin

MAP2

GFAP

 

DIV3

96W

384W

6.06 ± 3.34 (n = 608)

5.51 ± 3.45 (n = 155)

57.12 ± 14.03 (n = 661)

58.16 ± 7.14 (n = 904)

63.80 ± 8.12 (n = 608)

69.49 ± 5.07 (n = 882)

0.55 ± 30.61 (n = 661)

0.10 ± 0.30 (n = 904)

DIV7

96W

384W

1.36 ± 1.05 (n = 659)

n.a.

59.91 ± 16.55 (n = 695)

58.35 ± 6.20 (n = 999)

61.31 ± 7.59 (n = 659)

66.82 ± 9.47 (n = 738)

2.13 ± 1.36 (n = 695)

1.30 ± 1.04 (n = 999)

DIV14

96W

384W

3.36 ± 3.61 (n = 715)

2.84 ± 1.05 (n = 788)

59.45 ± 9.59 (n = 636)

62.54 ± 22.95 (n = 763)

87.29 ± 6.77 (n = 715)

71.53 ±19.17 (n = 788)

4.94 ± 1.48 (n = 636)

5.82 ± 2.65 (n = 763)

DIV21

96W

384W

5.36 ± 3.40 (n = 598)

6.00 ± 4.76 (n = 447)

68.38 ± 11.07 (n = 573)

80.89 ± 14.57 (n = 484)

90.80 ± 14.67 (n = 598)

98.76 ± 16.00 (n = 333)

7.70 ± 3.29 (n = 573)

9.22 ± 2.49 (n = 484)

  1. Data were evaluated by Attovision software analysing aNS-1 alive cells expressing neuronal (beta III-tubulin, MAP2), neural progenitor (Nestin) and glial (GFAP) markers; 96- and 384-well microplates experiments were run in parallel; n: number of cells analyzed.