Intrinsic response of thoracic propriospinal neurons to axotomy

BMC Neuroscience

Table 1 Animal and Experimental Treatment Groups

Animal Group	N	Label	Injury	Type	Survival Post Injury	Total Survival Time Post Label	Group Comparison
1	4	FG	No	---	---	10 Days	1 to 4
2	4	FG	No	---	---	3 Weeks	1 to 5
3	4	FG	No	---	---	5 Weeks	2 to 6
4	4	FG	Yes	TXN	3 Days	10 Days	3 to 7
5	4	FG	Yes	TXN	1 Week	2 Weeks
6	4	FG	Yes	TXN	2 Weeks	3 Weeks
7	4	FG	Yes	TXN	5 Weeks	5 Weeks
8	4	DTMR	Yes	CON	1 Week	2 Weeks	8 to 9
9	4	DTMR	No	---	---	2 weeks	8 to 5

Female Long-Evans rat were divided among 9 groups. Then using the indicated tracer, Fluorogold (FG) or Dextran Tetramethyl Rhodamine (DTMR), TPS neurons were prelabeled by a series of bilateral injections into the upper lumbrosacral enlargement. Following 1-week tracer transport time, animals in groups 4-8 were subjected to either a T9 complete spinal transection (TXN) or moderate spinal contusion injury (25 mm weight drop, NYU Impactor; CON). Animals were then allowed to recover for different periods post-injury as indicated. N used in analysis indicates the number of animals that were used in the data analysis part of this study. (*) Group 6 only has an N of 2 due to 1 animal failing to pass the microarray's internal quality control, and the loss of 1 animal to freezing artifact during histological processing. (*) Group 7 only has an N of 2 due to premature animal mortality. The final column of this table indicates the time point comparisons that were made for both the gene and PCR validation studies conducted during this study.

ISSN: 1471-2202