Figure 6

Immunofluorescent analysis of the TPS neuronal response to a low thoracic contusion injury 1-week post-injury. A, D, G, J, M, P, S, V , TPS neurons were retrogradely labeled by bilateral injections into the intermediate gray matter of the upper lumbosacral enlargement with DTMR 4 days prior to the injury, and 1-week following injury in cryostat sections (Cy3 Filter). B, E, H, K, N, Q, T, W , These sections also were immunolabeled by 1 of 8 antibodies. Examples show immuno-labeling within thoracic neurons (FITC Filter) related to cell survival, Hspb1; regeneration, Gap43; growth factor receptors, Gfra1, Ret, Ntrk1, Ntrk2, Ntrk3; and apoptosis, Casp3. C, F, I, L, O, R, U, X , TPS neurons colocalizing with the immuno-label for all 8 proteins are shown in these merged images. Y , Cartoons show the region of the intermediate gray matter from which the digital images were taken. All images taken at 40x; Scale bars = 100 μm.