Figure 5

PCR validation of gene expression data. A , The log2 difference was obtained to give the direction of change in the expression values. B , P values for the significance in change of expression were determined using a two-tailed student t-test, for each time point comparison. A, B , Values appearing both bolded and italicized are genes demonstrating a significant change in expression (P-value). F, A correlation analysis between the log2 differences of expression in gene array and PCR array was run to see if the expression trends observed in the gene array data corroborated the expression trends displayed in the PCR array. The overall correlation of 0.69 reveals a reasonable. C, D, E , The top three correlated genes (Hspb1, Gfra1, Gfra2) are plotted to illustrate the correlated expression trends between the log2 differences of the gene array data and PCR data. G , Summary of the data presented in panels A, B and F. Genes demonstrating a significant and correlated change in expression following injury in both the gene and PCR arrays are indicated in red (Actb, Fgf9, Gfra1, Hspb1). Genes with a significant change in expression in either the gene array or PCR array and demonstrate correlated expression trends are indicated in pink. Time points where no significant change in expression was found, but a correlation of expression between the two arrays is found are indicated in purple, The one time point where a significance in change of expression is found but the expression trends diverge is indicated in black.