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Figure 4 | BMC Neuroscience

Figure 4

From: Intrinsic response of thoracic propriospinal neurons to axotomy

Figure 4

Expression profiles of specific genes classes significantly altered following spinal transection. The expression profiles illustrate the normalized log2 expression values with low expression values being represented by green and high expression in red. The three expression profiles illustrated here were generated using a combination 2-factor ANVOA with a significance of (p = 0.01, based on 1000 permutations) and cluster analysis to reveal the expression patterns. A , Expression profiles for 14 of the 84 genes associated with regeneration (RAG) or cell survival and neuroprotection (CsNp) using the ANOVA analysis demonstrated a significant change in expression following injury. As shown, all were upregulated. B , The expression profile for genes associated with surface receptors, neurotrophic receptors, and neurotrophins and growth factors (GF). ANOVA analysis revealed 8 of the 214 genes examined exhibited a significant change in expression following the injury, 3 of which (Unc5a, Lingo1, Fgf9) are down-regulated. C , Even though both pro- and anti-apoptotic genes were included, all seven genes revealed by this analysis are pro-apoptotic. Only one of the seven genes is down regulated (Siva1), while the other 6 demonstrate a sharp up-regulation following axotomy. A, B, C All three-expression profiles have a common pattern in gene expression that matches the pattern found for the overall significant response (see Figure 2). Asterisk indicates genes from the genome wide hypothesis free analysis also identified in our specific gene programs. Plus sign indicates genes whose expression values were validated by qRT-PCR. Black circle indicates genes validated immunofluorescently.

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