Figure 1

Untreated and virus treated human embryonic spinal cord cells. A and B shows 8.5 weeks-old spinal cord cells incubated for 12 h with the Prv. In A, the human cell marker hu-NCAM is shown in red and GFP is green. In B, the GFAP+ cells are red and GFP is green. B shows embryonic spinal cord cells at higher magnification. The cord is poor in cells and the two types of immunolabelling can be clearly distinguished: there is no overlap between the GFP and the GFAP labellings. Note the relatively well-developed glial cells with extensive processes. In C, result of the mock-infection can be seen: Embryonic cells are positive for NCAM, but contain no GFP. Scale bar in A-C: 50 μm. D, E and F show 12-weeks-old untreated human embryonic spinal cord cells processed for GFAP and hu-NCAM double labelling immunohistochemistry. In D, the glial cells are green, and in E, the human cell marker hu-NCAM is red. In F, the merged image of D and E is shown. It should be noted, that the two types of immunolabelling do not overlap. The glial cells shown in E carry a radial glia-like morphology. Scale bar: 250 μm