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Table 1 Antibodies used for immunohistochemistry and Western blot analysis.

From: A short upstream promoter region mediates transcriptional regulation of the mouse doublecortin gene in differentiating neurons

Antibody

reference

Dilution

Company

  

ICC

IHC

WB

 

Anti-Dcx(C-18)

(Goat) sc-8066

1/200

1/500

1/1000

SantaCruz Biotechnology

Anti-Oct3/4 (C10)

(Mouse) sc-5279

1/200

 

1/200

SantaCruz Biotechnology

Anti-βIII Tubulin

(Mouse) T8660

1/1000

  

Sigma-Aldrich

Anti-α-Tubulin

(Mouse) T6074

  

1/25000

Sigma-Aldrich

Anti-NeuN

(Mouse) MAB377

1/100

1/500

 

Chemicon

TRITC-conjugated

(DAG) 705-025-147

1/500

1/500

 

Jackson Immuno Research

 

(DAM) 705-205-151

1/500

1/500

 

Jackson Immuno Research

HRP-conjugate

(RAG) A5420

  

1/2000

Sigma-Aldrich

 

(MAG) A4416

  

1/2000

Sigma-Aldrich