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Figure 7 | BMC Neuroscience

Figure 7

From: The TRPC2 channel forms protein-protein interactions with Homer and RTP in the rat vomeronasal organ

Figure 7

Whole-cell electrophysiology. (A-D) Human embryonic kidney 293 (HEK293) cells were transiently transfected with cDNAs encoding the TRPC2 channel, hCD8 and with or without RTP1 as noted. HEK293 cells were voltage-clamped at -60 mV in the whole-cell configuration and then a ramp was applied (+80 to -80 mV; see methods) under control (black) and subsequent to ATP bath application (red). (A) Representative control (beadless) whole-cell recording. The bottom recordings are of the entire voltage protocol. The box outlines the portions of these recordings shown in greater detail in the inset above the dashed lines and used in C-D. Representative recordings from the TRPC2 alone (B), TRPC2+ RTP1+ (C), and the heavily beaded TRPC2+ RTP1+ (D) conditions. (E) Graph of the change in mean (± s.e.m) current at -80 mV following ATP stimulation as compared to a previous stimulation under various transfection conditions as in (A-C). Solid black triangle = denotes the mean response to ATP stimulation by heavily beaded cells as in (D). Transfection sample size as noted. * = denotes mean current is statistically different from control transfection; ** = denotes mean current is statistically different from TRPC2 transfection; one-way ANOVA followed by a snk post-hoc test (p ≤ 0.05). The voltage-ramp protocol is described graphically in (A).

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