Figure 4
From: The TRPC2 channel forms protein-protein interactions with Homer and RTP in the rat vomeronasal organ
TRPC2 transfection efficiency in HEK293 cells. (A-H) Representative micrographs of cells grown on glass-coverslips, transfected as in text, incubated with an antiserum to the c-myc epitope and labeled with FITC-conjugated secondary antiserum. (A, C, E, and G) Representative fields of view for antiserum specific fluorescence (green). (B, D, F, and H) The same fields of view as in images B, D, F, and H with DAPI nuclear stain (blue). Note the low percentage of cells transfected in (A, B) TRPC2, (C, D) TRPC2 + RTP1, (E, F) TRPC2 + REEP1, as compared to (G, H) Kv1.3. The scale bar in G is 100 μm. (I) Histogram plot of the ratio of transfected cells. The number of quantified transfections are noted. * = denotes a mean pixel density significantly different from Kv1.3 transfection; one-way ANOVA followed by a snk post-hoc test (p ≤ 0.05).