Figure 1
From: The TRPC2 channel forms protein-protein interactions with Homer and RTP in the rat vomeronasal organ
Adaptor protein expression in the rat vomeronasal organ (VNO). (A-E) Representative Western blots of NP40-solubilized proteins incubated with Homer family antisera as labeled. Proteins were separated by SDS-PAGE and then electro-transferred to nitrocellulose as described in the text. Expected Mr = 45/47, 29 kDa. Abbreviations: H = hippocampus, VNO = vomeronasal organ, OB = olfactory bulb, Ce = cerebellum, CH = cerebral hemispheres, T = testis, M = male, F = female. Representative of 3-4 individual preparations for each antiserum. (F) Two representative trials of RT-PCR products specific for RTP1 (RTP) and REEP1 (REEP), respectively. The expected PCR products were 597 bp (RTP1) and 623 bp (REEP1). CON = no reverse transcriptase negative control.