Curcumin reduces intracellular ROS and cytotoxicity of transiently transfected WTαS-EGFP SH-SY5Y cells. Localization of WTαS-EGFP and protective effects of curcumin in transiently transfected SH-SY5Y cells. (A) Representative images of WTαS-EGFP transfected cells in the absence (i-iii) and presence of curcumin (iv-vi) were captured using a Nikon TE300 fluorescence microscope. Scale bar = 50 μm. (B) ROS levels and (C) cytotoxicity of the untransfected control, αS-transfected and αS-transfected+curcumin were measured. For LDH assay, cell culture media was collected after 48 hr and the LDH release was expressed as a percentage of the untransfected samples. For ROS measurements, DCFH was added to each well and the DCF fluorescence was measured at Ex485 nm and Em535 nm after an additional incubation at 37°C for 30 min. DCF fluorescence was expressed as a percentage of the untransfected control reported as mean ± SD, n = 3. **p < 0.01; ***p < 0.001, compared with the untransfected control, #p < 0.05; ###p < 0.001 compared with αS-transfected sample analyzed using one way ANOVA followed by Bonferroni post-hoc test.