Sleep and lifespan in Hk1 and wild-type siblings reared and tested at 25°C. A. Twenty-four hour values of sleep and waking activity from eclosure to death and survival curves. Circles represent significant differences (Mann-Whitney test; p < 0.05) B. Sleep parameters (24-hour values) at different ages. C. Sleep parameters during night and day. Age range and number of flies are shown below each bar. Genotype (g) effects were tested across all ages (Kruskal-Wallis test, p < 0.05). Horizontal bars indicate significant age-related effects within each genotype (Friedman test followed by Wilcoxon signed-rank test, p < 0.05). D. Time course of sleep parameters in 30-min bins. Comparisons (Friedman test followed by Wilcoxon signed-rank test, p < 0.05) are between the 10-20 day period and all following ages: triangle (vs 35-45), circle (vs 60-70), square (vs 85-95).