Levels of expression of Spg7, Afg3l1 and Afg3l2. A. Real time RT-PCR measures, calibrated relative to the housekeeping gene Pgk1. Bars represent standard errors. B. Real-time RT-PCR analysis of Afg3l2 and Spg7 transcripts in the whole cerebellum and in pools of Purkinje cells. The expression levels are relative to the 18S rRNA.