In-situ hybridization staining for Spg7, Afg3l1 and Afg3l2 in cerebellum. Panels on the left show overviews of cerebella cut along sagittal planes. Panels on the right show the central portion of cerebella at a higher magnification. Note the intense staining of Purkinje cells and of neurons of deep cerebellar nuclei. The molecular layer is devoid of labeling. Abbreviations: ml, molecular layer; gl, granular layer; pcl and arrow, Purkinje cell layer. Calibration bars are 250 μm for left column and 125 μm for right column panels.