In-situ hybridization staining for Spg7 , Afg3l1 and Afg3l2 in hippocampus and neocortex. A. Hippocampus. Pyramidal neurons and dentate gyrus are labelled with all three antisense probes. Note that interneurons selectively express Afg3l2. Abbreviations: so, stratum oriens; sr, stratum radiatum; slm, stratum lacunosum-moleculare; h, hilus; DG, dentate gyrus; CA1, CA3, hippocampal regions cornu ammonis 1 and 3. B. In-situ hybridization staining for Spg7, Afg3l1 and Afg3l2 in neocortex. Sections of neocortex are shown from the pial surface (top) to the border between layer VI and white matter (bottom). With the exception of layer I, which contains only few cells, all layers display a homogeneous distribution of labelled cells, with a higher staining of layer V. In the left panel, cortical layers are labelled from I to VI. Calibration bars are 125 μm.