Figure 2
Expression of genes of interest in RTT and control samples, individual samples. The expression of genes of interest was measured by quantitative RT-PCR. Expression of each transcript was evaluated in six RTT and six control frontal and occipital cortices, and normalized to the expression of GAPDH in the same sample. RTT = Rett syndrome; CTL = control; FC = frontal cortex and OC = occipital cortex. Samples that were expressed more than 10-fold higher than housekeeping genes were serially diluted and multiple dilutions were analyzed. The Y-axis shows the ratio of the transcript of interest normalized against GAPDH (a) APLP1 mRNA expression levels in RTT FC were significantly higher than in control FC (p = 0.02), but not compared to RTT OC (p = 0.06); (b) CLU mRNA expression levels in RTT FC were significantly higher than in control FC (p = 0.042) and RTT OC (p = 0.047); (c) CO1 mRNA expression levels in RTT FC were significantly lower than in control FC (p = 0.024) and RTT OC (p = 0.024); (d) CRMP1 mRNA expression levels in RTT FC were significantly higher than in control FC (p = 0.049) and RTT OC (p = 0.045); (e) DNMI mRNA expression levels in RTT FC were significantly greater than in the control frontal cortex (p = 0.048) and RTT OC (p = 0.004), however expression in the RTT OC was significantly lower than in the control OC (p = 0.004); (f) EML2 mRNA expression levels in RTT FC were not significantly different to the RTT OC (p = 0.062), or the control FC (p = 0.213); (g) GDI1 expression in the RTT FC was significantly greater than the RTT OC (p = 0.045), but not compared to expression in the control FC (p = 0.14); (h) GNBI mRNA expression in the RTT FC was significantly greater than in the RTT OC (p = 0.026), but not compared to the control OC (p = 0.164); (i) HUM mRNA expression in the RTT FC was not significantly greater than in the RTT OC (0.064) or the control FC (p = 0.092); (j) RTN3 mRNA expression in the RTT FC was not significantly greater than in the RTT OC (p = 0.148) or the control FC (p = 0.378).