Figure 1From: Cerebrospinal fluid promotes survival and astroglial differentiation of adult human neural progenitor cells but inhibits proliferation and neuronal differentiationSurvival rate of human adult NSCs during expansion. A, The percentage of necrotic NSCs expanded in culture medium supplemented with EGF and FGF-2 in comparison to CSF with or without 150 ng/ml Noggin was determined 72 hours after starting the expansion process. Values represent the mean ± S.E.M from at least three independent experiments. * indicates P < 0.05 and ** P < 0.01 compared to each corresponding value in CSF. B, Representative microphotographs demonstrating the influence of CSF on survival rate of NSCs. For immunostainings, dead cells were stained with propidiumiodid. Nuclei were counterstained with Hoechst 33342. The upper panel shows NSCs expanded in CSF (left photograph) and CSF+Noggin (right photograph), the lower panel shows NSCs expanded in expansion media supplemented with EGF and FGF. Scale bar = 50 μmBack to article page