Zebra finch HSD17B4 gene and cDNA clone identities. (A) HSD17B4 gene model and EST structure based on the zebra finch genome assembly. The 5' end of the gene is to the left; a scale of the Z chromosome positions is on the bottom in 0.01 Mb increments. The gene model shows the coding exon structure. The zebra finch EST CK313884 is mapped below the entire gene model to show that the probes used in the study span approximately half of the gene at the 3' end. (B) Alignment of zebra finch EST CK313884 predicted protein sequence to that of chicken, human, mouse and zebra fish. Overall, there is high conservation of the amino acid sequence across species, including two functional domains: the hydratase-dehydrogenase epimerase (HDE, dashed box) domain and sterol carrier protein 2 (SCP2, solid box) domain, demonstrating the specificity of the probes used in molecular experiments.