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Figure 1 | BMC Neuroscience

Figure 1

From: N-arachidonoyl glycine, an abundant endogenous lipid, potently drives directed cellular migration through GPR18, the putative abnormal cannabidiol receptor

Figure 1

NAGly-induced directed BV-2 microglial migration. (A) BV-2 microglial migration in response to basal conditions; vh (0.1% DMSO); 1 μM fMLP; 1 μM LPA; 0.1 nM - 300 μM NAGly. * = P < 0.05, ** = P < 0.01 compared to 1 μM fMLP; one-way ANOVA; n = 8. Insert is a filter photograph of one random field of view at ×40 magnification indicating the migration produced by 100 nM NAGly. The 10 μm diameter pores can be discerned as the clear unstained circles. (B) BV-2 microglial migration in response to basal conditions; vh (0.1% DMSO); 1 μM fMLP ± concentration gradient; 0.1 nM - 10 μM NAGly ± concentration gradient. ** = P < 0.01 compared to the corresponding concentration gradient; Student's unpaired t-test; n = 3. Insert is a filter photograph of one random field of view at ×40 magnification indicating the migration produced by 100 nM NAGly in the absence of a concentration gradient.

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