Figure 1
From: α-Synuclein overexpression increases dopamine toxicity in BE(2)-M17 cells
Expression levels of α-synuclein in stably transfected M17 cell line. Immunoblot of α-synuclein in cell lines overexpressing WT or A30P α-synuclein using a monoclonal antibody against α-synuclein (upper panel). The same immunoblot was then stripped and reprobed with an antibody for β-actin (lower panel). Quantitation of α-synuclein is expressed as a ratio between the major α-synuclein band and β-actin. Cells transfected with vector alone show moderate expression of α-synuclein compared to cells overexpressing WT or A30P α-synuclein.