Figure 2
From: Nonmuscle Myosin II helps regulate synaptic vesicle mobility at the Drosophilaneuromuscular junction
Quantification of NMMII alleles. Western blot of NMMII alleles a, Knockdown (K/D) of NMMII in the nervous system reduced NMMII expression to 28% (n = 5, P < 0.05) whereas the heterozygous loss-of-function zip1/CyO (Het) reduced NMMII expression to 57% (n = 4, P < 0.05) compared to the elav3AGal4 control (ct). Overexpression of zipGS50077(O/E) in the nervous system increased NMMII expression by 95% (n = 4, P < 0.05) compared to the elav3AGal4 control (ct). α-β-Tubulin was used as the loading control. Relative NMMII proteins levels were quantified from the western blot analysis. Each band was first normalized to the level of β-tubulin (used as a loading control, not shown) and then the mutant genotypes were normalized to elav3AGal4 protein levels, which were set to 1. The bars indicate the mean level obtained from 4-5 samples, bars are SEM. Overexpression (O/E), knockdown (K/D), heterozygous loss-of-function (Het).